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--- methods:novelobjectlocationrecognition [2023/07/14 10:46] – ohardt
+++ methods:novelobjectlocationrecognition [2023/07/15 15:19] (current) – external edit 127.0.0.1
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 If the animal recognizes that the object moved to a new location, we expect it to explore it more than the object at the familiar location.
+Similar to the novel object recognition task, many variables of this protocol can be modified, such as the number of trials in each phase and their dureation, memory retention interval, how many objects are in the open field, and how many of them are moved.
+We record all phases on video with cameras mounted above the open field.  There are never other animals or an experimenter in the room while a rat is in the open field during a trial.  Trained observers score animal behaviour, i.e., the time they explore objects, from the recorded videos.  The data are then used to calculate the novelty preference index DI=[(time exploring object at novel location)-(time exploring object at familiar location)]/(time exploring both).  DI=0 indicates that both object locations were explored the same, i.e., the absence of preference or the behaviour that would be expected by chance alone.  Di>0 indicates that the object moved to a novel location was explored more than the one that did not change its position, and DI<0 indicates the opposite.  To determine whether DI is significantly different from what would be expected by chance alone, a one-sample t-test comparing DI against 0 can be used.
+Our detailed protocols are available at the SOP section.