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methods:sop:methazolamide

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methods:sop:methazolamide [2023/10/31 10:29] ohardtmethods:sop:methazolamide [2023/10/31 10:29] (current) ohardt
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 ==== Preparing Stock Solution ==== ==== Preparing Stock Solution ====
-- Molecular weight of methazolamide is 236.47 +  - Molecular weight of methazolamide is 236.47 
-- Stock concentration: 100 mM +  - Stock concentration: 100 mM 
-- Take 50 mg methazolamide and add 2.1143 mL of DSMO +  - Take 50 mg methazolamide and add 2.1143 mL of DSMO 
-- Aliquot into 20 µL aliquots and freeze in -80 C freezer+  - Aliquot into 20 µL aliquots and freeze in -80 C freezer
  
 ==== Preparing Infusion Solution ==== ==== Preparing Infusion Solution ====
-- Dilute Stock to final concentration of 10 mM +  - Dilute Stock to final concentration of 10 mM 
-- Take one 20 µL aliquot and thaw it +  - Take one 20 µL aliquot and thaw it 
-- Add 180 µL DSMO to aliquot +  - Add 180 µL DSMO to aliquot 
-- Briefly (3 s) vortex+  - Briefly (3 s) vortex
  
methods/sop/methazolamide.1698762553.txt.gz · Last modified: 2023/10/31 10:29 by ohardt