methods:sop:methazolamide
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methods:sop:methazolamide [2023/10/31 10:29] – ohardt | methods:sop:methazolamide [2023/10/31 10:29] (current) – ohardt | ||
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==== Preparing Stock Solution ==== | ==== Preparing Stock Solution ==== | ||
- | - Molecular weight of methazolamide is 236.47 | + | |
- | - Stock concentration: | + | - Stock concentration: |
- | - Take 50 mg methazolamide and add 2.1143 mL of DSMO | + | - Take 50 mg methazolamide and add 2.1143 mL of DSMO |
- | - Aliquot into 20 µL aliquots and freeze in -80 C freezer | + | - Aliquot into 20 µL aliquots and freeze in -80 C freezer |
==== Preparing Infusion Solution ==== | ==== Preparing Infusion Solution ==== | ||
- | - Dilute Stock to final concentration of 10 mM | + | |
- | - Take one 20 µL aliquot and thaw it | + | - Take one 20 µL aliquot and thaw it |
- | - Add 180 µL DSMO to aliquot | + | - Add 180 µL DSMO to aliquot |
- | - Briefly (3 s) vortex | + | - Briefly (3 s) vortex |
methods/sop/methazolamide.1698762553.txt.gz · Last modified: 2023/10/31 10:29 by ohardt